September 30, 2012
Observe thebone morphogenetic protein 4 (BMP4) isolated primary cultured rat brain stem cells (NSC) differentiation induced by cholinergic effect. From 2-month-old rat hippocampus, striatum region isolated cells were cultured in DMEM/F12 medium containing EGF and bFGF, to Nestin (of nestin in parallel morphological characteristics of the cells in the light microscope,) cytochemical staining. After 24h, a switch of BMP4-containing culture medium, the culture was continued for 7 or 8 days. Morphological changes of the cells in the light microscope, parallel choline acetyltransferase (choline acetyltransferase staining of ChAT) and nestin double-labeled immune cells. The results showed that, plus BMP4 cultured for 7 or 8 days, the light microscope to see 34% of the cultured cells with the morphological characteristics of neurons. The immunocytochemical staining visible ChAT-positive cells and nestin-positive cells coexist ChAT-positive cells accounted for 28% of nestin-positive cells accounted for 38%. In short, in the culture broth was added the BMP4 can induce NSC differentiation into cells having a cholinergic properties
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