September 30, 2012

Bone morphogenetic protein 4 on the stem cells of the rat brain choline induced differentiation effect

Observe thebone morphogenetic protein 4 (BMP4) isolated primary cultured rat brain stem cells (NSC) differentiation induced by cholinergic effect. From 2-month-old rat hippocampus, striatum region isolated cells were cultured in DMEM/F12 medium containing EGF and bFGF, to Nestin (of nestin in parallel morphological characteristics of the cells in the light microscope,) cytochemical staining. After 24h, a switch of BMP4-containing culture medium, the culture was continued for 7 or 8 days. Morphological changes of the cells in the light microscope, parallel choline acetyltransferase (choline acetyltransferase staining of ChAT) and nestin double-labeled immune cells. The results showed that, plus BMP4 cultured for 7 or 8 days, the light microscope to see 34% of the cultured cells with the morphological characteristics of neurons. The immunocytochemical staining visible ChAT-positive cells and nestin-positive cells coexist ChAT-positive cells accounted for 28% of nestin-positive cells accounted for 38%. In short, in the culture broth was added the BMP4 can induce NSC differentiation into cells having a cholinergic properties

Posted by: BMP4protein at 01:51 AM | No Comments | Add Comment
Post contains 177 words, total size 1 kb.

September 10, 2012

BMP4 only bone injury repair related

In this experiment, we fracture repair process a BMP4mRNA Expression and localization of detected using BMP4cDNA probe, in order to investigate their role in fracture healing. It was found that: (1) not detected in normal bone tissue and soft tissue and BMP4mRNA expression; (2) early fracture (fracture after 12 to 72 hours) that a BMP4mRNA expression; (3) BMP4 gene mainly in early fracture hematoma emerging within the cell and fracture the surrounding soft tissue, expressed in mesenchymal cells. Consistent with the the Nakase other reported results. Bone marrow stromal cells may be derived from these hematoma, osteoblast potential of stem cells, i.e. directed into the bone precursor cells, the emerging positive cells in muscle tissue compared can induce osteogenic precursor cells, i.e. mesenchymal cells.

Trauma to activate the BMP4 gene's expression and showed some regional, that is limited to the fracture callus formation around the area of soft tissue. Topical application of exogenous recombinant expression product BMP4 able to induce new bone formation in normal soft tissue ectopic, so they think, local BMP4 gene expression of the fracture hematoma the osteogenic ability is the very important factor in the process of fracture healing callus formation also shows that the fracture hematoma and soft tissue has a very important position in the process of fracture healing. BMP4 gene expression is also reported in the literature in embryonic tissue, organs and embryonic bone, endochondral bone repair fractures embryonic osteogenesis reproduction, BMP4 only bone injury repair related, are more likely to tissues and organs and embryos related.

Posted by: BMP4protein at 12:17 PM | No Comments | Add Comment
Post contains 260 words, total size 2 kb.

September 04, 2012

Histological changes in the process of fracture healing is a complex and involve a number of different cell activities

Histological changes in the process of fracture healing is a complex and involve a number of different cell activities. Fracture occurs, the presence of the different stages of osteoblast and osteoclast number and their synthetic cell interstitial far can not meet the demand of the fracture healing. Local cell regulation mechanisms (including precursor cells, mesenchymal cells, other synergy cells, capillaries, lymphatic, nerve growth factor autocrine and paracrine) to produce a sufficient number of osteoblasts and osteoclasts.

With the development of recombinant DNA technology, making it possible therapeutic areas. Such as growth factors and some other regulatory peptides for the regulation of cell metabolism, division and differentiation in the bone healing process. Part in the process of fracture healing growth factors and cytokines involved in the formation of bone cartilage has been reported in the literature.

Posted by: BMP4protein at 06:53 AM | No Comments | Add Comment
Post contains 154 words, total size 1 kb.

August 29, 2012

Demonstration and Localization of Bone Morphogenetic Protein-4( BMP4) in Fracture Healing

Post-traumatic bone tissue regeneration and repair is very complete, and its repair is often no scar residue. The reason why there is such a perfect bone tissue healing ability, because the memory of bone tissue factor in successful bone, bone growth factors. Bone morphogenetic proteins (bone morphogenetic pro-tein, BMP) because of its emphasis on direct induction of soft tissue into bone. Application BMP4cDNA probe to detect the fracture healing process foreign callus localization and distribution of BMP4 gene expression to explore the BMP4 gene expression outside in closed fracture healing callus formation. 64 healthy SD rats with closed tibial fractures animal models. 12 hours after fracture, 1,3,5,7,9,14 and 28 days after operation. Drawn underwent cryostat, the digoxigenin labeled BMP4cDNA probe situ hybridization. Rat fracture after 12 hours to three days, the detection of fractures around hematoma within cells and muscle emerging mesenchymal cells BMP4mRNA expression as a positive signal. Show that The trauma activates expression of BMP4mRNA, and was regional involved in the repair of fractures, the fracture hematoma and soft tissue has a very important role in the process of fracture healing.


Posted by: BMP4protein at 02:40 PM | No Comments | Add Comment
Post contains 194 words, total size 1 kb.

August 27, 2012

Bone morphogenetic protein can be induced to enhance the osteogenic potential of bone marrow and allogeneic bone

Follow-up period of 3 months to 6 years, with an average of 44 months follow-up of more than 3 years 28 patients (36 hips), 18 patients (22 hips) F ic cases at stage I, II, hip no significant pain and dysfunction, more than 3 years follow-up of 28 patients (36 hips) the hip Ha rris score, the mean preoperative (4 ± 9) minutes, mean postoperative (7 ± 7. 2) points (P <0. 0 1). The authors believe that the method of using bone morphogenetic protein can induce to enhance the osteogenic potential of bone marrow and allogeneic bone allograft fibula to provide direct mechanical support for block development and collapse of the femoral head lesions, bone allograft bone morphogenetic protein and autologous bone marrow can be used as the treatment of femoral F ic at , of avascular necrosis of a choice.

Posted by: BMP4protein at 07:02 AM | No Comments | Add Comment
Post contains 160 words, total size 1 kb.

August 22, 2012

the expression level directly affect the future into the bone's blood supply, affect the speed and quality of the osteoblast.

Angiogenesis is an important part of the fracture healing process, a good blood supply to the fracture site of great significance in fracture repair. Vascular endothelial growth factor (vascular endothelial growth factor, VEGF) is a multifunctional cytokine, one of the most directly promotion of angiogenesis research has shown to play an important role of VEGF in bone formation and bone metabolism, the role is mainly by promoting angiogenesis involved in bone development in the form. Many recent studies have shown that VEGF is mainly derived from vascular endothelial cells is an important mediator of angiogenesis induced by multiple cytokines. On the other hand, increased expression levels of these cytokines will lead to increased expression of VEGF or synthetic. Various cytokines to promote bone efficacy and VEGF expression in varied ways, better application of what kind of cytokines, or combined with the feasibility of a variety of cytokines, there is no clear conclusion.

VEGF is a multifunctional cytokine secreted by platelets, megakaryocytes, endothelial cells and osteoblasts, and its role throughout human reproduction, development, tissue regeneration, repair, and tumor pathophysiological process. Many recent studies have shown that also play an important role of VEGF in bone formation and repair, the expression level directly affect the future into the bone's blood supply, affect the speed and quality of the osteoblast.

Posted by: BMP4protein at 03:05 PM | No Comments | Add Comment
Post contains 237 words, total size 2 kb.

July 29, 2012

BMP, bFGF are two very important cytokines in bone formation

BMP, bFGF, has long been studies have shown that bone formation in two very important cytokines. BMP has long been proved to have a strong osteoinductive capacity. From bone cells themselves synthesized bFGF in an autocrine or paracrine in the bone matrix, combining bFGF and osteoblast-like cell surface receptor can promote its conversion to osteogenic cells. In vitro experiments confirmed that of bFGF can directly stimulate rat calvaria bone cells increased DNA synthesis, thereby increasing the number of osteoblasts the synthesis of collagen. Recently, studies have found that BMP, bFGF, and VEGF secretion and expression of the existence of a very close relationship. This experiment on the basis of the results of previous studies, observation of VEGF, respectively, in the application of rhBMP-2 expression and distribution of bFGF stimulation of bone marrow stromal cells, and the corresponding performance for osteogenesis do a comparison and found that in vitro application of rhBMP-2 and bFGF can indeed promote the expression of important mediators of VEGF to promote vascular endothelial cell proliferation, while increasing osteoblast performance. The joint application of these two cytokines can not only accelerate the bone marrow stromal cell proliferation and promote cell conversion to osteoblasts direction, so as to achieve better osteogenesis. First of bFGF to promote the proliferation of bone marrow stromal cells and then apply to promote the expression of osteogenic phenotype rhBMP-2, then the effect on the proliferation of bFGF and rhBMP-2 to promote differentiation are unaffected, which can play two cytokines in maximum biological effectiveness.

Posted by: BMP4protein at 01:43 PM | No Comments | Add Comment
Post contains 260 words, total size 2 kb.

VEGF was the introduction of bone tissue engineering technology

VEGF is a multifunctional cytokine secreted by platelets, megakaryocytes, endothelial cells and osteoblasts, and its role throughout human reproduction, development, tissue regeneration, repair, and tumor pathophysiological process. Many recent studies have shown that also play an important role of VEGF in bone formation and repair, the expression level directly affect the future into the bone's blood supply, affect the speed and quality of the osteoblast.

VEGF was the introduction of bone tissue engineering technology is bound to promote new bone formation, increase the speed and quality of new bone. Exogenous VEGF in vivo half-life is short, and expensive, its application bring great inconvenience. Bone marrow stromal cells is a commonly used seed cells for bone tissue engineering, it contains many cells such as endothelial cells into fiber cells, macrophages, etc. can be secreted expression of VEGF in. How to stimulate the bone marrow stromal cells to promote the secretion of VEGF is another important means of expression of VEGF

Posted by: BMP4protein at 01:40 PM | No Comments | Add Comment
Post contains 168 words, total size 1 kb.

Angiogenesis is an important part of the fracture healing process

In the early stage of fracture, soft tissue defects, systemic disease causes new blood vessels will lead to delayed union or nonunion. Bone after transplantation, the activity of vascular granulation tissue ingrowth of bone grafts, bone graft is absorbed, take the initiative to the formation of new bone. However, in the large segment of bone grafts, this process slow and incomplete. The key to these problems lies in the revascularization of bone problems that plagued mankind for a long time.

Angiogenesis is an important part of the fracture healing process, a good blood supply to the fracture site of great significance in fracture repair. Vascular endothelial growth factor (vascular endothelial growth factor, VEGF) is a multifunctional cytokine, one of the most directly promotion of angiogenesis research has shown to play an important role of VEGF in bone formation and bone metabolism, the role is mainly by promoting angiogenesis involved in bone development in the form. Many recent studies have shown that VEGF is mainly derived from vascular endothelial cells is an important mediator of angiogenesis induced by multiple cytokines. On the other hand, increased expression levels of these cytokines will lead to increased expression of VEGF or synthetic. Various cytokines to promote bone efficacy and VEGF expression in varied ways, better application of what kind of cytokines, or combined with the feasibility of a variety of cytokines, there is no clear conclusion.

Posted by: BMP4protein at 01:38 PM | No Comments | Add Comment
Post contains 242 words, total size 2 kb.

May 26, 2012

Isolation, Culture and Cholinergic-Induced Differentiation with BMP4 of Adult Rats’ Neural Stem Cells

Research has confirmed that neural stem cells have the ability to update itself and multiplex differentiation potential. Different cell factors can affect the proliferation and differentiation of neural stem cells in the process. Neural stem cells of neurons can express a variety of neurotransmitters, such as GABA, substance P, dopamine, etc, but did not add in special induction, most of the neural stem cells to differentiate into nature can GABA neurons, spontaneous grow into other phenotype neurons is very rare. Many studies show that by adjusting the cell factors can change the differentiation direction, neural stem cell differentiation for a specific type of cell. Some reports BMP 9 (BMP9) can by inducing acetylcholine shift enzyme and acetylcholine transport sac of gene expression, and increase the training of nerve cells acetylcholine synthesis; Brain BMP9 can increase indoor injection on live animals in the brain tissue of acetylcholine levels, think BMP9 is the central nervous system of the choline can differentiation factor. BMP in the family of neural stem cells with BMP4 whether the choline can differentiation and raise the nerve cells choline can express effect, has not been seen experiment report. This experiment through training and somatic cells in the body from animals know whether it has the role BMP4. First of all, from 2 months of SD rats hippocampus, the striatum, the third ventricle and the lateral ventricle master's degree thesis of neural stem cells in adult rats separation cultivation and BMP4 acetylcholine differentiation VZ, SVZ area to separate neural stem cells. The mechanical scattered and chemical digest method separating out of the cell, serum-free medium, with training; To Nestin, NF160 and GFAP antibodies as an antioxidant, immune cells chemical dyeing. The results showed that in optical differ under a microscope to see the cultured cells have the neural stem cells form characteristics and proliferation properties; Immune cells cultured cells chemical dyeing saw a Nestin positive reaction, NF 1 60, GFAP negative reaction, namely cell have neural stem cells in the immunological characteristics. Then, the neural stem cells in the medium to join or not join BMP4, observe the choline can differentiation condition. Will train the neural stem cells into two groups, BMP4 group to switch to join BMP4 induction medium, training, no BMP4 group still using the original medium training as a comparison; Eight days after training to cultivate cells were performed respectively ChAI... Antibody indirect immunofluorescence stain, ChAT antibodies and Nestin antibody SABC a DS double standard immune cells dyeing and mark of chemical FITC choline can flow cytometry detection.
The results show a more cells BMP4 group strong green fluorescent, show ChAT positive; And no BMP4 group less cells produce a weak ChAT fluorescence. Immune cell chemical dyeing of bids are three kinds of cells: one kind is ChAT antibody response positive cell, it is purple and blue cell plasma, and some of the cells are typical neurons morphological characteristics; The second Nestin antibody response positive cells, the red cell plasma, a round or sample precursor cells the shape of the cells in the part of the cells in process of division; The third kind for Nestin and ChAI, antibody response negative cells, the cells in the slurry not shading, cells have neurons morphological characteristics. Microscope count, BMP4 group of cell, ChAT reaction positive about 28%, Nestin reaction of positive about 38%; And no BMP4 group of cells, Ch door a, positive reaction of about 8%, and the positive reaction Nestin is about 52%. Flow cytometry BMP4 group ChAT was testing positive cells significantly higher than no rate BMP4 group (16%, 7%, P < 0. 05). The results show that: the generation of BMP4 separation, rats in vitro culture in the hippocampus and the striatum of the neural stem cells have such as choline can differentiation entrainment. Third, will BMP4 injection into adult rats in the hippocampus right the dentate gyrus to nerve cells to observe its master's degree thesis of neural stem cells in adult rats separation cultivation and BMP4 acetylcholine differentiation choline can express influence. Will only 20 adult male SD rats randomly divided into two groups: control group BMP4 group and salt water. After anesthesia, using three-dimensional localization technology will 1 wind BMP4 (BMP4 group) or physiological saline injection (control group) rat hippocampal right the dentate gyrus area. After 14 days take brain, paraffin slice, do ChAT antibody immunohistochemical study. With image analysis system calculation rat hippocampal left or right side the dentate gyrus ChAT positive cells of the total area, with 51, Al 'A 7.0 t test software. The results show positive neurons in the hippocampus ChAT CAI, CAZ, CA3 and the dentate gyrus all have distribution; BMP4 rats in the hippocampus right the dentate gyrus district door corpse positive cells Ch area significantly higher than the left (P < 0.01); Whereas the control rat hippocampal left or right side the dentate gyrus ChAT positive cells within the area of no significant difference (P > 0.05). Two groups of comparisons, BMP4 rats in the hippocampus in the right the dentate gyrus ChAI... Positive cells is significantly higher than the control group of the same parts (P < 0.01); And the left between the two groups in the hippocampus in the dentate gyrus ChAI, positive cells no significant difference in the area (P >.05). The results indicated that, the hippocampus the dentate gyrus area after the injection BMP4 accept, can induce the cells appear higher in the choline can express. In short, the results of this study indicate that: (l) from an adult rat striatum hippocampus, such as isolated area has morphological characteristics of neural stem cells and immunological characteristics of cells; (2) BMP4 join in the medium to induce the in vitro culture adult rats, the hippocampus striatum area such as neural stem cells in the choline can differentiation; (3) the hippocampus BMP4 into the dentate gyrus adult rats to induce in the neurons choline can express hike. Tip of the clan has induced BMP4 BMP neural stem cells in the choline can express or the effects of differentiation, worth in neural degenerative diseases of diseases such as animal experiment and clinical treatment for further study

Posted by: BMP4protein at 01:21 PM | No Comments | Add Comment
Post contains 1040 words, total size 7 kb.

<< Page 1 of 1 >>
35kb generated in CPU 0.02, elapsed 0.063 seconds.
30 queries taking 0.0495 seconds, 61 records returned.
Powered by Minx 1.1.6c-pink.